LOCUS       NM_003140                828 bp    mRNA    linear   PRI 03-JUN-2022
DEFINITION  Homo sapiens sex determining region Y (SRY), mRNA.
ACCESSION   NM_003140
VERSION     NM_003140.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Kherouatou-Chaoui N, Chellat-Rezgoune D, Rezgoune ML, Mc Elreavey K,
            Touabti LS, Abadi N and Satta D.
  TITLE     SRY and NR5A1 gene mutation in Algerian children and adolescents
            with DSD and testicular dysgenesis
  JOURNAL   Afr Health Sci 21 (3), 1491-1497 (2021)
   PUBMED   35222615
  REMARK    GeneRIF: SRY and NR5A1 gene mutation in Algerian children and
            adolescents with DSD and testicular dysgenesis.
REFERENCE   2  (bases 1 to 828)
  AUTHORS   Calvo SE, Pagliarini DJ and Mootha VK.
  TITLE     Upstream open reading frames cause widespread reduction of protein
            expression and are polymorphic among humans
  JOURNAL   Proc Natl Acad Sci U S A 106 (18), 7507-7512 (2009)
   PUBMED   19372376
  REMARK    GeneRIF: Includes the study of a disease-related upstream ORF in
            this gene, and shows that it functions to reduce protein levels by
            100%.
REFERENCE   3  (bases 1 to 828)
  AUTHORS   Sekido R and Lovell-Badge R.
  TITLE     Sex determination and SRY: down to a wink and a nudge?
  JOURNAL   Trends Genet 25 (1), 19-29 (2009)
   PUBMED   19027189
  REMARK    Review article
REFERENCE   4  (bases 1 to 828)
  AUTHORS   Clepet C, Schafer AJ, Sinclair AH, Palmer MS, Lovell-Badge R and
            Goodfellow PN.
  TITLE     The human SRY transcript
  JOURNAL   Hum Mol Genet 2 (12), 2007-2012 (1993)
   PUBMED   8111368
REFERENCE   5  (bases 1 to 828)
  AUTHORS   Behlke MA, Bogan JS, Beer-Romero P and Page DC.
  TITLE     Evidence that the SRY protein is encoded by a single exon on the
            human Y chromosome
  JOURNAL   Genomics 17 (3), 736-739 (1993)
   PUBMED   8244390
REFERENCE   6  (bases 1 to 828)
  AUTHORS   Su H and Lau YF.
  TITLE     Identification of the transcriptional unit, structural organization,
            and promoter sequence of the human sex-determining region Y (SRY)
            gene, using a reverse genetic approach
  JOURNAL   Am J Hum Genet 52 (1), 24-38 (1993)
   PUBMED   8434602
REFERENCE   7  (bases 1 to 828)
  AUTHORS   Mohnach,L., Fechner,P.Y. and Keegan,C.E.
  TITLE     Nonsyndromic Disorders of Testicular Development
  JOURNAL   (in) Adam MP, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH, Gripp KW
            and Amemiya A (Eds.); GENEREVIEWS((R)); (1993)
   PUBMED   20301714
REFERENCE   8  (bases 1 to 828)
  AUTHORS   Delot,E.C. and Vilain,E.J.
  TITLE     Nonsyndromic 46,XX Testicular Disorders/Differences of Sex
            Development
  JOURNAL   (in) Adam MP, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH, Gripp KW
            and Amemiya A (Eds.); GENEREVIEWS((R)); (1993)
   PUBMED   20301589
REFERENCE   9  (bases 1 to 828)
  AUTHORS   Hawkins JR, Taylor A, Berta P, Levilliers J, Van der Auwera B and
            Goodfellow PN.
  TITLE     Mutational analysis of SRY: nonsense and missense mutations in XY
            sex reversal
  JOURNAL   Hum Genet 88 (4), 471-474 (1992)
   PUBMED   1339396
REFERENCE   10  (bases 1 to 828)
  AUTHORS   Sinclair AH, Berta P, Palmer MS, Hawkins JR, Griffiths BL, Smith MJ,
            Foster JW, Frischauf AM, Lovell-Badge R and Goodfellow PN.
  TITLE     A gene from the human sex-determining region encodes a protein with
            homology to a conserved DNA-binding motif
  JOURNAL   Nature 346 (6281), 240-244 (1990)
   PUBMED   1695712
COMMENT     ##Evidence-Data-START##
            Transcript is intronless :: L10101.1, BC074923.2 [ECO:0000345]
            ##Evidence-Data-END##
            ##RefSeq-Attributes-START##
            MANE Ensembl match       :: ENST00000383070.2/ ENSP00000372547.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from L10101.1.
            This sequence is a reference standard in the RefSeqGene project.
            On May 17, 2019 this sequence version replaced NM_003140.2.
            Summary: This intronless gene encodes a transcription factor that
            is a member of the high mobility group (HMG)-box family of
            DNA-binding proteins. This protein is the testis-determining factor
            (TDF), which initiates male sex determination. Mutations in this
            gene give rise to XY females with gonadal dysgenesis (Swyer
            syndrome); translocation of part of the Y chromosome containing
            this gene to the X chromosome causes XX male syndrome. [provided by
            RefSeq, Jul 2008].
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /map="Yp11.2"
     gene            1..828
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="sex determining region Y"
                     /db_xref="GeneID:6736"
                     /db_xref="HGNC:HGNC:11311"
                     /db_xref="MIM:480000"
     exon            1..828
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    20..22
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="upstream in-frame stop codon"
     CDS             80..694
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="essential protein for sex determination in human
                     males; sex-determining region on Y; testis-determining
                     factor on Y; truncated sex-determining region Y protein"
                     /codon_start=1
                     /product="sex-determining region Y protein"
                     /protein_id="NP_003131.1"
                     /db_xref="CCDS:CCDS14772.1"
                     /db_xref="GeneID:6736"
                     /db_xref="HGNC:HGNC:11311"
                     /db_xref="MIM:480000"
                     /translation="MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQ
                     CETGENSKGNVQDRVKRPMNAFIVWSRDQRRKMALENPRMRNSEISKQLGYQWKMLTEA
                     EKWPFFQEAQKLQAMHREKYPNYKYRPRRKAKMLPKNCSLLPADPASVLCSEVQLDNRL
                     YRDDCTKATHSRMEHQLGHLPPINAASSPQQRDRYSHWTKL"
     misc_feature    254..487
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Sufficient for interaction with KPNB1.
                     /evidence=ECO:0000269|PubMed:15297880"
     misc_feature    260..310
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Required for nuclear localization.
                     /evidence=ECO:0000269|PubMed:12764225,
                     ECO:0000269|PubMed:15746192"
     misc_feature    398..496
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Sufficient for interaction with EP300.
                     /evidence=ECO:0000269|PubMed:15297880"
     misc_feature    467..487
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Required for nuclear localization.
                     /evidence=ECO:0000269|PubMed:15297880"
     misc_feature    485..487
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000269|PubMed:15297880; propagated from
                     UniProtKB/Swiss-Prot (Q05066.1); acetylation site"
     misc_feature    491..544
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Necessary for interaction with ZNF208 isoform
                     KRAB-O. /evidence=ECO:0000269|PubMed:15469996"
     misc_feature    602..691
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    671..691
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Necessary for interaction with SLC9A3R2.
                     /evidence=ECO:0000269|PubMed:9054412"
     regulatory      807..812
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="hexamer: AATAAA"
     polyA_site      828
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="major polyA site"
ORIGIN
        1 agaagtgagt tttggatagt aaaataagtt tcgaactctg gcacctttca attttgtcgc
       61 actctccttg tttttgacaa tgcaatcata tgcttctgct atgttaagcg tattcaacag
      121 cgatgattac agtccagctg tgcaagagaa tattcccgct ctccggagaa gctcttcctt
      181 cctttgcact gaaagctgta actctaagta tcagtgtgaa acgggagaaa acagtaaagg
      241 caacgtccag gatagagtga agcgacccat gaacgcattc atcgtgtggt ctcgcgatca
      301 gaggcgcaag atggctctag agaatcccag aatgcgaaac tcagagatca gcaagcagct
      361 gggataccag tggaaaatgc ttactgaagc cgaaaaatgg ccattcttcc aggaggcaca
      421 gaaattacag gccatgcaca gagagaaata cccgaattat aagtatcgac ctcgtcggaa
      481 ggcgaagatg ctgccgaaga attgcagttt gcttcccgca gatcccgctt cggtactctg
      541 cagcgaagtg caactggaca acaggttgta cagggatgac tgtacgaaag ccacacactc
      601 aagaatggag caccagctag gccacttacc gcccatcaac gcagccagct caccgcagca
      661 acgggaccgc tacagccact ggacaaagct gtaggacaat cgggtaacat tggctacaaa
      721 gacctaccta gatgctcctt tttacgataa cttacagccc tcactttctt atgtttagtt
      781 tcaatattgt tttcttttct ctggctaata aaggccttat tcatttca
//
LOCUS       NG_011751               7897 bp    DNA     linear   PRI 31-JAN-2022
DEFINITION  Homo sapiens sex determining region Y (SRY), RefSeqGene on
            chromosome Y.
ACCESSION   NG_011751
VERSION     NG_011751.1
KEYWORDS    RefSeq; RefSeqGene.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Calvo SE, Pagliarini DJ and Mootha VK.
  TITLE     Upstream open reading frames cause widespread reduction of protein
            expression and are polymorphic among humans
  JOURNAL   Proc Natl Acad Sci U S A 106 (18), 7507-7512 (2009)
   PUBMED   19372376
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Sekido R and Lovell-Badge R.
  TITLE     Sex determination and SRY: down to a wink and a nudge?
  JOURNAL   Trends Genet 25 (1), 19-29 (2009)
   PUBMED   19027189
  REMARK    Review article
REFERENCE   3  (bases 1 to 7897)
  AUTHORS   Clepet C, Schafer AJ, Sinclair AH, Palmer MS, Lovell-Badge R and
            Goodfellow PN.
  TITLE     The human SRY transcript
  JOURNAL   Hum Mol Genet 2 (12), 2007-2012 (1993)
   PUBMED   8111368
REFERENCE   4  (bases 1 to 7897)
  AUTHORS   Behlke MA, Bogan JS, Beer-Romero P and Page DC.
  TITLE     Evidence that the SRY protein is encoded by a single exon on the
            human Y chromosome
  JOURNAL   Genomics 17 (3), 736-739 (1993)
   PUBMED   8244390
REFERENCE   5  (bases 1 to 7897)
  AUTHORS   Su H and Lau YF.
  TITLE     Identification of the transcriptional unit, structural organization,
            and promoter sequence of the human sex-determining region Y (SRY)
            gene, using a reverse genetic approach
  JOURNAL   Am J Hum Genet 52 (1), 24-38 (1993)
   PUBMED   8434602
REFERENCE   6  (bases 1 to 7897)
  AUTHORS   Mohnach,L., Fechner,P.Y. and Keegan,C.E.
  TITLE     Nonsyndromic Disorders of Testicular Development
  JOURNAL   (in) Adam MP, Ardinger HH, Pagon RA, Wallace SE, Bean LJH, Gripp KW,
            Mirzaa GM and Amemiya A (Eds.); GENEREVIEWS((R)); (1993)
   PUBMED   20301714
REFERENCE   7  (bases 1 to 7897)
  AUTHORS   Delot,E.C. and Vilain,E.J.
  TITLE     Nonsyndromic 46,XX Testicular Disorders of Sex Development
  JOURNAL   (in) Adam MP, Ardinger HH, Pagon RA, Wallace SE, Bean LJH, Gripp KW,
            Mirzaa GM and Amemiya A (Eds.); GENEREVIEWS((R)); (1993)
   PUBMED   20301589
REFERENCE   8  (bases 1 to 7897)
  AUTHORS   Sinclair AH, Berta P, Palmer MS, Hawkins JR, Griffiths BL, Smith MJ,
            Foster JW, Frischauf AM, Lovell-Badge R and Goodfellow PN.
  TITLE     A gene from the human sex-determining region encodes a protein with
            homology to a conserved DNA-binding motif
  JOURNAL   Nature 346 (6281), 240-244 (1990)
   PUBMED   1695712
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006040.3.
            This sequence is a reference standard in the RefSeqGene project.
            Summary: This intronless gene encodes a transcription factor that
            is a member of the high mobility group (HMG)-box family of
            DNA-binding proteins. This protein is the testis-determining factor
            (TDF), which initiates male sex determination. Mutations in this
            gene give rise to XY females with gonadal dysgenesis (Swyer
            syndrome); translocation of part of the Y chromosome containing
            this gene to the X chromosome causes XX male syndrome. [provided by
            RefSeq, Jul 2008].
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /map="Yp11.2"
     gene            complement(2313..2946)
                     /gene="RNASEH2CP1"
                     /gene_synonym="AYP1p1"
                     /note="ribonuclease H2 subunit C pseudogene 1"
                     /pseudo=""
                     /db_xref="GeneID:386608"
                     /db_xref="HGNC:HGNC:24117"
     gene            5070..5897
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="sex determining region Y"
                     /db_xref="GeneID:6736"
                     /db_xref="HGNC:HGNC:11311"
                     /db_xref="MIM:480000"
     mRNA            5070..5897
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /product="sex determining region Y"
                     /transcript_id="NM_003140.3"
                     /db_xref="GeneID:6736"
                     /db_xref="HGNC:HGNC:11311"
                     /db_xref="MIM:480000"
     exon            5070..5897
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /inference="alignment:Splign:2.1.0"
                     /number=1
     CDS             5149..5763
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="essential protein for sex determination in human
                     males; sex-determining region on Y; testis-determining
                     factor on Y; truncated sex-determining region Y protein"
                     /codon_start=1
                     /product="sex-determining region Y protein"
                     /protein_id="NP_003131.1"
                     /db_xref="CCDS:CCDS14772.1"
                     /db_xref="GeneID:6736"
                     /db_xref="HGNC:HGNC:11311"
                     /db_xref="MIM:480000"
                     /translation="MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQ
                     CETGENSKGNVQDRVKRPMNAFIVWSRDQRRKMALENPRMRNSEISKQLGYQWKMLTEA
                     EKWPFFQEAQKLQAMHREKYPNYKYRPRRKAKMLPKNCSLLPADPASVLCSEVQLDNRL
                     YRDDCTKATHSRMEHQLGHLPPINAASSPQQRDRYSHWTKL"
     misc_feature    5323..5556
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Sufficient for interaction with KPNB1.
                     /evidence=ECO:0000269|PubMed:15297880"
     misc_feature    5329..5379
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Required for nuclear localization.
                     /evidence=ECO:0000269|PubMed:12764225,
                     ECO:0000269|PubMed:15746192"
     misc_feature    5467..5565
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Sufficient for interaction with EP300.
                     /evidence=ECO:0000269|PubMed:15297880"
     misc_feature    5536..5556
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Required for nuclear localization.
                     /evidence=ECO:0000269|PubMed:15297880"
     misc_feature    5554..5556
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000269|PubMed:15297880; propagated from
                     UniProtKB/Swiss-Prot (Q05066.1); acetylation site"
     misc_feature    5560..5613
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Necessary for interaction with ZNF208 isoform
                     KRAB-O. /evidence=ECO:0000269|PubMed:15469996"
     misc_feature    5671..5760
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    5740..5760
                     /gene="SRY"
                     /gene_synonym="SRXX1; SRXY1; TDF; TDY"
                     /note="propagated from UniProtKB/Swiss-Prot (Q05066.1);
                     Region: Necessary for interaction with SLC9A3R2.
                     /evidence=ECO:0000269|PubMed:9054412"
ORIGIN
        1 tgaccttcat tttatggaga gaaacaagct ataacatgta gtatctaagc tgattagaag
       61 aactaaaaag agaagctcat acttgtgcat cagaaggtaa atgaaagagt gaagttacct
      121 ctttgtttta aggaagaaag gaaaattgtg gatgtcatct gttttctgtt tacatatttc
      181 aggcatggat agccacaatg tgattttaag acggttagtt acaactgatt tgaaaaaaaa
      241 aaaaaatgct tcactctatg agaaatttct tcccaagtat gaaaccttgt ttttacaggc
      301 aatttcctat actttgaaaa aatcaaaata ataaagtaaa agaaaaataa ttcaggtgaa
      361 gttagagaaa aaaacaggca gcattatttt aaagttgtaa actattttgt ttacttatag
      421 tttaatttac atgtagtaga tatgcatttg taaggttctt cggctcaggt aggagatcat
      481 tctatttccc actgcaccct acttcatcct cccactggca aataattaga ttatccctgg
      541 gaaaaaaaga tgccagtaaa attgatcatg tttaaatgca tcagttgcta ggtgatttat
      601 ctgattaagt cttgaaacag tagaacctag caattaaagt gagcattaac ttctacctac
      661 caaatcagaa gactattcta actttttgag aattagatgt tgaaaatatg gcccatgaat
      721 ttagcatggt taaaataaaa aacatgcaaa caaaacaaac ccaacatctt gaaaggacat
      781 ttgactctaa agtcccaaaa ataatcacaa gtctaaaaat cctaagttta gtgttactct
      841 attacacctt tttatttgta agtgtccttt cacaaaagtt ttaaattttg ctcttgtgca
      901 ttttatttac cttttctttt gttgtttgtg tctttggtga cctgccaacc attagacttc
      961 aaaaaacagc ctatagccaa gctgcaggat aaatgaacac ataagttgac ttagaatagt
     1021 caactctgtc tagtatacaa tttatggggg atggtttatg accacatata tttctacttt
     1081 gatgggaata tcttgagata aaattagaga gaatgagtgg agtaatattc acaacatttt
     1141 tgctgcattc atccctgaat ttgaagaaat accaaagtac atcttgtgag gagaaaaaat
     1201 aaataaattc atataaaatg ttgtgggttt tattctttat gcagtggtaa actgtgtttg
     1261 catacaccat agcaattaaa ttagggctac aaagggtatt taactaatga gcataaaata
     1321 ccttaatgta cctcaaatgc aattaattgc attggaccaa tctaagttac tattcttcag
     1381 ttttcatttt tatttcatta ttcatttcat ttttattctg atataaaaat gaaccaggat
     1441 ctgtgtgaaa ttatttgaat ctaatgtctt tgaacatttt tcttaccata ccttaagatt
     1501 aaaaaaacaa aaaaaaatcc cttagtttgg caacttttgc tgttggttaa gcccgtttgg
     1561 atttaacatt gacaggacca gctaacttcc taccagttaa cattgcttgt gggcctgact
     1621 gatgtaaaaa tatttaacaa ctgttgagaa atagtcatca gtgaatctta aaggtcgtac
     1681 tcatataaaa caatatagcg ctttaacttt ttatttactg aatttgagag aaaggaatag
     1741 tgttccagaa actgggatca ctcaggcgct cagggaagca tatggttagc tgatgatata
     1801 aaggcccaat attggaagaa ctgttaatgt agtcccctgg agacagtgag tgaattatta
     1861 gccctggttg agagattaaa atgtgttgat atgatattat agaggcaaat ttaatgctct
     1921 cggcctcccc ctgcatcctt atttttgcat gtaggtttat tactttacag gagaaccaac
     1981 acttttttcc tttttttttt tttaagtggt tgagtttgtt tttctgaacc cagaggcatg
     2041 tttacacatt tcattaattt aagtaccaga gaagtatact gagaacctac tacatgtcac
     2101 acactatcct aggagctgta gacagaaaaa taataaaatc tcgtgtcttt atttaaattt
     2161 tgttgactgc cacccccaag tgagtctcct cacacacccc gtcctgtaat atatgcatct
     2221 gggaggtctt ttttgccttc ttaaaaacat atagatggtt ggacatatgt atataagaat
     2281 ataaaattca ccactttatc ttttgtgaat gtgtgctgtg aagaactcct ttactggggt
     2341 gatggaacca gtggctacaa agtaaggagc tggtttactg ctgtaaaggg ttcgcggctt
     2401 tgaatttcaa gctctggttc tgtgtccttg ggcacctgcg cgtgaatcgt tgccgcgagg
     2461 ctgggccaag ttaaggcccc acgcagtttg gcttccgggc caaggaagcc ccacagggtg
     2521 ccccacaggg tgaagcccca tgccctacag ggtgaagcgg ctgaagctgg tagtggttcc
     2581 gaggaagcgg tcaaagtccc gctccagagg ttcctcttct tggttgtcac tcccggaacc
     2641 ccgccagggg tctggcttcc ccatcgacac ctcctcctgt tcagtcacca tcacgtatcc
     2701 cacgaggccg gacggcactg ccacctcgtc gccccgtaga ctgcggcccc gaaactacac
     2761 ttaagagtcc ctcggggccc tggcggatgg caggcgtgat gaagcgccac accgtggtgg
     2821 gccgcagggt atcaggtgta gtgtggcggg ggcggcgtcc tgcagtgtgg ctgggcgcaa
     2881 ctggacgctg tacctctcca tagccgccta gtcgccgctc tccatcctcg ctcctgtgcg
     2941 acgccacttt atccctctta aactggacga ttcagtagta ccggggaacg aaggcaacaa
     3001 gacctaacca tagcaacaac attttgtttt tgaaacccat cttgcaactg cctgactgca
     3061 aaatgtactt gcagattctg cagacaaaaa ttgctgccac ttattaaaag acttcatagt
     3121 gaaaactgac tttttaccca aaccactgat tcaaggactt ttgcgtagaa actcatgaac
     3181 tgttaagata acccttcatt accaattaag ataataggtt gtaagtaacc taatgagtac
     3241 caactttctc ttatttcctt taaaaactgt ctctcaaaac agccggtcac gacacaatgt
     3301 cagatgcttc tggaatctgt ttctctaatt acagttctaa aactgccaac aaactcctta
     3361 tttggacttc agtttctctg actctttggt tcaccatgtt gtgcagccat cacctctctc
     3421 tagttccaga gcatatttta tcaatcctca aaagaaaccg tgcatccacc agcagtaact
     3481 ccccacaacc tctttcatcc agtccttagc aaccattaat ctggttttag tctctattca
     3541 tttgcctttc ctggatattt catatacatg ggatcattca gtatctggcc tcttgtatct
     3601 gactttttca cacagtgtaa cattttcaag gttcacctat gtggtgcctt gtgtcatttt
     3661 ttgctatttt catgtcaggt tttgaaatgg tatgtttttt ctcatcttgt aagggatttc
     3721 ctgttgtaga aaatctgttt ctgtgtatga ctgcaattta accattttat ttgctatcca
     3781 gatacatgtt ttaacttgag ccaactatat ttccactttg tataatgaat actaaatttt
     3841 gtatcttaga tactaaactt tagtagataa ctaggataca aattacagtt ttcatatttt
     3901 tcttttcatg gaatataatt ttttaaagtt tctgatatta attcattata acatttactg
     3961 gacaaaccaa ccacatttgg ttctatgaac tcttttcata ttttacggga tttttatttt
     4021 gttgtcttag gaattgtggc atcattcagt tttgctgaga gtttattttt attaataggt
     4081 tctgtgtact atgcaacctg tagttgttac tcctgttttg caaatatggc attaaaacat
     4141 tggtgagtgg ggggactttc ctcccatttt atttcagcaa agaaatcttg tcatttctct
     4201 tcagacttta agatttattg aggagagtta ttcacttgtt tagtctggta aactgtgacc
     4261 tttaaatttt gaaggaggtt gaactaaaag gtgaaatatg ataaattgat ggctctattt
     4321 gttgtgaatg gaaatttaat ttagacttgt aaaagattgt tttttaaatt gctaattgtg
     4381 tgacagtgaa gagaatgact tcaaaatatc ccactataca atctgcaaag aaaagaattg
     4441 tgtccccttt ttagtgtagc ttaacacttc actgaaactg ttttgagttc ttaggtcata
     4501 tttttttttc tctaaacgaa acaattactt ttctaaaagt caaatgttag ccatcctaga
     4561 agttgggcat aaaatacttg taagtatatg ctaatattct gatacttaat gcctgtgaaa
     4621 aatgtgtata gaattttcaa tttttaaata gaagtgaaga aaaagcgata ataattacta
     4681 taaattcaat atgcagttat gtatgtatgt gtgtggttaa gacaattagg ttctcattaa
     4741 gctttgtttt tttaaagata acatacacat atattgataa tgataaacaa ttcatatagc
     4801 tttttgtgtc ctctcgtttt gtgacataaa aggtcaatga aaaaattggc gattaagtca
     4861 aattcgcatt tttcaggaca gcagtagagc agtcagggag gcagatcagc agggcaagta
     4921 gtcaacgtta ctgaattacc atgttttgct tgagaatgaa tacattgtca gggtactagg
     4981 gggtaggctg gttgggcggg gttgaggggg tgttgagggc ggagaaatgc aagtttcatt
     5041 acaaaagtta acgtaacaaa gaatctggta gaagtgagtt ttggatagta aaataagttt
     5101 cgaactctgg cacctttcaa ttttgtcgca ctctccttgt ttttgacaat gcaatcatat
     5161 gcttctgcta tgttaagcgt attcaacagc gatgattaca gtccagctgt gcaagagaat
     5221 attcccgctc tccggagaag ctcttccttc ctttgcactg aaagctgtaa ctctaagtat
     5281 cagtgtgaaa cgggagaaaa cagtaaaggc aacgtccagg atagagtgaa gcgacccatg
     5341 aacgcattca tcgtgtggtc tcgcgatcag aggcgcaaga tggctctaga gaatcccaga
     5401 atgcgaaact cagagatcag caagcagctg ggataccagt ggaaaatgct tactgaagcc
     5461 gaaaaatggc cattcttcca ggaggcacag aaattacagg ccatgcacag agagaaatac
     5521 ccgaattata agtatcgacc tcgtcggaag gcgaagatgc tgccgaagaa ttgcagtttg
     5581 cttcccgcag atcccgcttc ggtactctgc agcgaagtgc aactggacaa caggttgtac
     5641 agggatgact gtacgaaagc cacacactca agaatggagc accagctagg ccacttaccg
     5701 cccatcaacg cagccagctc accgcagcaa cgggaccgct acagccactg gacaaagctg
     5761 taggacaatc gggtaacatt ggctacaaag acctacctag atgctccttt ttacgataac
     5821 ttacagccct cactttctta tgtttagttt caatattgtt ttcttttctc tggctaataa
     5881 aggccttatt catttcagtt ttactggtat ttcattttaa acttaatttc aagacaagtt
     5941 gtgtcaacac gattaacatg caaagaaata agacatccag aagtgagcct gcctatgttt
     6001 gtggccgtca gagtactaac ttgatacaaa cggacactgt ggcttacttt aaatgctcta
     6061 atgagaaaca cacttgaaaa ttgtaccaaa aaaaatcaca cttctatatg cagcgtgtta
     6121 agcagtcctc tctagaccgt gtattcattg gtctttcagc tactttgtac gtgtctataa
     6181 attgcaggta actaaggaat ggatatgtaa gcaggatcaa acttgtttct ttctctcccc
     6241 ttcacgctgt ggaaaaaacc agttttacct ccacttgcaa ttcagttcct ttactccata
     6301 taaatccaaa cggttgacat ttcctttcaa ctagttataa aatgcctctg gtaaaacaaa
     6361 atatttaatt ccttgtcatt tttgtatctc tatgaaactt atcattttgc ctttcttctg
     6421 aaaactatct tttaaaatgg caatctactt gtttccatgg cctattaact tttaagcctg
     6481 tggaatgaaa aatacagaat tttcattctc aacaggaatg ttgagaacgc cgactaccca
     6541 gattatggag atggaagggc gggggctggt gacccaactg gtttaatttg atgggattta
     6601 aataaaaatg taatgccaag acttcataaa atttgcacat aagctaaagc aggaaactag
     6661 aagtttacaa ataactgtaa accacagttt aaagtataaa ttacaaataa atttttctac
     6721 aataaaaata aatcaaaagt caaaattaat ggtatgtatc aaagtcagct ttccaatatt
     6781 tcaaaacttt tcagagacat taccttaaat aataaaactc aaacactaaa agagtgtata
     6841 ttagagatgg atgtcatgtt tctcaaagtt cagggtttaa aaaaagcttt attccagaga
     6901 caatattact taatctgagc tttatgccaa atgagaactg tatattgccc attacctttt
     6961 aaacactgac tcaaaactac tttgtaatat ttaaaaccac tcttttcact ctcccaaaaa
     7021 taacacccat tctgaccttc attaccatac attaattttc cttttcttga acctctgaca
     7081 cagattctct gtttgaccaa actttagtca ggtttctgaa ccttctccta gactcgcctg
     7141 tgcacttcct tgccaaactc agttttggca aagaactctc ctaagccagg ttagccagac
     7201 tgtaccccca ccgcatatct gatcacactc aacacttgat cgatcaccct caatatctga
     7261 tcaggtttct catcctccac caccctctag gtgatgtctg atcagcctag cctgtcttca
     7321 gcaagaatcc tccttatcta tgatgtcttc tcttagtgat tgtccatcca ttgaccctac
     7381 tccctgctcc ttggctataa atccccacct acccagctgt gtatgtggag ctaaggccaa
     7441 tctctccccc agtgcaagac ctcattgctg tgctgttggt cgtgaatagt cttccttatc
     7501 aagtttgaac aagtatcact gaataccatt tttccttaac aatgtgaata aatataaata
     7561 caaaatatta tgtggagctt cttttgctta atacattttg gtggttttca gattattaaa
     7621 aattatgaca tcatgcatgt atacattttg gaaagactaa atcaagctaa tgtatctact
     7681 acctcatata cttccctttc cttttgcact gggaacattt aaattgtatt ctcttagcaa
     7741 ttttcaagca tacaatacat tcttattacc tatattcaac atgctttaca atagatctga
     7801 actaattgct tctatctaga tgaaatatta taatttttga ataacaatac tcatctcttc
     7861 acctgaccct actagagggt agccacaatt ctactcc
//
LOCUS       MK011519                 625 bp    mRNA    linear   PRI 11-FEB-2019
DEFINITION  Homo sapiens isolate P13-3 sex-determining region Y protein (SRY)
            mRNA, complete cds.
ACCESSION   MK011519
VERSION     MK011519.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Functional characterization of SRY variants in Azoospermic patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 625)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) Department of Molecular Reproduction
            Development and Genetics, Indian Institute of Science, near D Gate,
            Bangalore, Karnataka 560012, India
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /isolate="P13-3"
                     /isolation_source="azoospermic patient"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /sex="male"
                     /tissue_type="testis"
                     /country="India"
                     /PCR_primers="fwd_name: sry2f, fwd_seq:
                     cggatccttgacaatgcaatcatatgcttc, rev_name: sry2r, rev_seq:
                     cctcgagtcctacagctttgtccag"
     gene            1..625
                     /gene="SRY"
     CDS             11..625
                     /gene="SRY"
                     /codon_start=1
                     /product="sex-determining region Y protein"
                     /protein_id="QBA69876.1"
                     /translation="MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQ
                     CETGENSKGNVQDRVKRPMNAFIVWSRDQRRKMALENPRMRNSEISKQPGYQWKMLTEA
                     EKWPFFQEAQKLQAMHREKYPNYKYRPRRKAKMLPKNCSLLPADPASVLCSEVQLDNRL
                     YRDDCTKATHSRMEHQLGHLPPINAASSPQQRDRYSHWTKL"
ORIGIN
        1 atgcaatcat atgcaatcat atgcttctgc tatgttaagc gtattcaaca gcgatgatta
       61 cagtccagct gtgcaagaga atattcccgc tctccggaga agctcttcct tcctttgcac
      121 tgaaagctgt aactctaagt atcagtgtga aacgggagaa aacagtaaag gcaacgtcca
      181 ggatagagtg aagcgaccca tgaacgcatt catcgtgtgg tctcgcgatc agaggcgcaa
      241 gatggctcta gagaatccca gaatgcgaaa ctcagagatc agcaagcagc cgggatacca
      301 gtggaaaatg cttactgaag ccgaaaaatg gccattcttc caggaggcac agaaattaca
      361 ggccatgcac agagagaaat acccgaatta taagtatcga cctcgtcgga aggcgaagat
      421 gctgccgaag aattgcagtt tgcttcccgc agatcccgct tcggtactct gcagcgaagt
      481 gcaactggac aacaggttgt acagggatga ctgtacgaaa gccacacact caagaatgga
      541 gcaccagcta ggccacttac cgcccatcaa cgcagccagc tcaccgcagc aacgggaccg
      601 ctacagccac tggacaaagc tgtag
//
LOCUS       MK011518                 622 bp    mRNA    linear   PRI 11-FEB-2019
DEFINITION  Homo sapiens isolate P12-1 sex-determining region Y protein (SRY)
            mRNA, partial cds.
ACCESSION   MK011518
VERSION     MK011518.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Functional characterization of SRY variants in Azoospermic patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 622)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) Department of Molecular Reproduction
            Development and Genetics, Indian Institute of Science, near D Gate,
            Bangalore, Karnataka 560012, India
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /isolate="P12-1"
                     /isolation_source="azoospermic patient"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /sex="male"
                     /tissue_type="testis"
                     /country="India"
                     /PCR_primers="fwd_name: sry2f, fwd_seq:
                     cggatccttgacaatgcaatcatatgcttc, rev_name: sry2r, rev_seq:
                     cctcgagtcctacagctttgtccag"
     gene            1..>622
                     /gene="SRY"
     CDS             11..>622
                     /gene="SRY"
                     /codon_start=1
                     /product="sex-determining region Y protein"
                     /protein_id="QBA69875.1"
                     /translation="MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQ
                     CETGENSKGNVQDRVKRPMNAFIVWSRDQRRKMALENPRMRNSEISKQLGYQWKMLTEA
                     EKWPFFQEAQKLQAMHREKYPNYKYRPRRKAKMLPKNCSLLPADPASVLRSEVQLDNRL
                     YRDDCTKATHSRMEHQLGHLPPINAASSPQQRDRYSHWTKL"
ORIGIN
        1 atgcaatcat atgcaatcat atgcttctgc tatgttaagc gtattcaaca gcgatgatta
       61 cagtccagct gtgcaagaga atattcccgc tctccggaga agctcttcct tcctttgcac
      121 tgaaagctgt aactctaagt atcagtgtga aacgggagaa aacagtaaag gcaacgtcca
      181 ggatagagtg aagcgaccca tgaacgcatt catcgtgtgg tctcgcgatc agaggcgcaa
      241 gatggctcta gagaatccca gaatgcgaaa ctcagagatc agcaagcagc tgggatacca
      301 gtggaaaatg cttactgaag ccgaaaaatg gccattcttc caggaggcac agaaattaca
      361 ggccatgcac agagagaaat acccgaatta taagtatcga cctcgtcgga aggcgaagat
      421 gctgccgaag aattgcagtt tgcttcccgc agatcccgct tcggtactcc gcagcgaagt
      481 gcaactggac aacaggttgt acagggatga ctgtacgaaa gccacacact caagaatgga
      541 gcaccagcta ggccacttac cgcccatcaa cgcagccagc tcaccgcagc aacgggaccg
      601 ctacagccac tggacaaagc tg
//
LOCUS       MK011517                 622 bp    mRNA    linear   PRI 11-FEB-2019
DEFINITION  Homo sapiens isolate P4-8 sex-determining region Y protein (SRY)
            mRNA, partial cds.
ACCESSION   MK011517
VERSION     MK011517.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Functional characterization of SRY variants in Azoospermic patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 622)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) Department of Molecular Reproduction
            Development and Genetics, Indian Institute of Science, near D Gate,
            Bangalore, Karnataka 560012, India
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /isolate="P4-8"
                     /isolation_source="azoospermic patient"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /sex="male"
                     /tissue_type="testis"
                     /country="India"
                     /PCR_primers="fwd_name: sry2f, fwd_seq:
                     cggatccttgacaatgcaatcatatgcttc, rev_name: sry2r, rev_seq:
                     cctcgagtcctacagctttgtccag"
     gene            1..>622
                     /gene="SRY"
     CDS             11..>622
                     /gene="SRY"
                     /codon_start=1
                     /product="sex-determining region Y protein"
                     /protein_id="QBA69874.1"
                     /translation="MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQ
                     CETGENSKGNVQDRVKRPMNAFIVWSRDQRRKMALENPRMRNSEISKQLGYQWKMLTEA
                     EKWPFFQEAQKLQAMHREKYPNYRYRPRRKAKMLPKNCSLLPADPASVLCSEVQLDNRL
                     YRDDCTKATHSRMEHQLGHLPPINAASSPQQRDRYSHWTKL"
ORIGIN
        1 atgcaatcat atgcaatcat atgcttctgc tatgttaagc gtattcaaca gcgatgatta
       61 cagtccagct gtgcaagaga atattcccgc tctccggaga agctcttcct tcctttgcac
      121 tgaaagctgt aactctaagt atcagtgtga aacgggagaa aacagtaaag gcaacgtcca
      181 ggatagagtg aagcgaccca tgaacgcatt catcgtgtgg tctcgcgatc agaggcgcaa
      241 gatggctcta gagaatccca gaatgcgaaa ctcagagatc agcaagcagc tgggatacca
      301 gtggaaaatg cttactgaag ccgaaaaatg gccattcttc caggaggcac agaaattaca
      361 ggccatgcac agagagaaat acccgaatta taggtatcga cctcgtcgga aggcgaagat
      421 gctgccgaag aattgcagtt tgcttcccgc agatcccgct tcggtactct gcagcgaagt
      481 gcaactggac aacaggttgt acagggatga ctgtacgaaa gccacacact caagaatgga
      541 gcaccagcta ggccacttac cgcccatcaa cgcagccagc tcaccgcagc aacgggaccg
      601 ctacagccac tggacaaagc tg
//
LOCUS       MK011516                 625 bp    mRNA    linear   PRI 11-FEB-2019
DEFINITION  Homo sapiens isolate P3-9 sex-determining region Y protein (SRY)
            mRNA, complete cds.
ACCESSION   MK011516
VERSION     MK011516.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Functional characterization of SRY variants in Azoospermic patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 625)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) Department of Molecular Reproduction
            Development and Genetics, Indian Institute of Science, near D Gate,
            Bangalore, Karnataka 560012, India
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /isolate="P3-9"
                     /isolation_source="azoospermic patient"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /sex="male"
                     /tissue_type="testis"
                     /country="India"
                     /PCR_primers="fwd_name: sry2f, fwd_seq:
                     cggatccttgacaatgcaatcatatgcttc, rev_name: sry2r, rev_seq:
                     cctcgagtcctacagctttgtccag"
     gene            1..625
                     /gene="SRY"
     CDS             11..625
                     /gene="SRY"
                     /codon_start=1
                     /product="sex-determining region Y protein"
                     /protein_id="QBA69873.1"
                     /translation="MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQ
                     CETGENSKGNVQDRVKRPMNAFIVWSRDQRRKMALENPRMRNSEISKQLGYQWKMLTEA
                     EKWPFFQEAQKLQAMHREKYPNYKYRPRRKAKMLPKNCSLLPADPASVLCSEVQLDNRL
                     YRDDCTKATHSRMEHQLGHLPPINAASSPQQRDRYSHWTKL"
ORIGIN
        1 atgcaatcat atgcaatcat atgcttctgc tatgttaagc gtattcaaca gcgatgatta
       61 cagtccagct gtgcaagaga atattcccgc tctccggaga agctcttcct tcctttgcac
      121 tgaaagctgt aactctaagt atcagtgtga aacgggagaa aacagtaaag gcaacgtcca
      181 ggatagagtg aagcgaccca tgaacgcatt catcgtgtgg tctcgcgatc agaggcgcaa
      241 gatggctcta gagaatccca gaatgcgaaa ctcagagatc agcaagcagc tgggatacca
      301 gtggaaaatg cttactgaag ccgaaaaatg gccattcttc caggaggcac agaaattaca
      361 ggccatgcac agagagaaat acccaaatta taagtatcga cctcgtcgga aggcgaagat
      421 gctgccgaag aattgcagtt tgcttcccgc agatcccgct tcggtactct gcagcgaagt
      481 gcaactggac aacaggttgt acagggatga ctgtacgaaa gccacacact caagaatgga
      541 gcaccagcta ggccacttac cgcccatcaa cgcagccagc tcaccgcagc aacgggaccg
      601 ctacagccac tggacaaagc tgtag
//
LOCUS       MK011515                 625 bp    mRNA    linear   PRI 11-FEB-2019
DEFINITION  Homo sapiens isolate P3-6 sex-determining region Y protein (SRY)
            mRNA, complete cds.
ACCESSION   MK011515
VERSION     MK011515.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Functional characterization of SRY variants in Azoospermic patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 625)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) Department of Molecular Reproduction
            Development and Genetics, Indian Institute of Science, near D Gate,
            Bangalore, Karnataka 560012, India
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /isolate="P3-6"
                     /isolation_source="azoospermic patient"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /sex="male"
                     /tissue_type="testis"
                     /country="India"
                     /PCR_primers="fwd_name: sry2f, fwd_seq:
                     cggatccttgacaatgcaatcatatgcttc, rev_name: sry2r, rev_seq:
                     cctcgagtcctacagctttgtccag"
     gene            1..625
                     /gene="SRY"
     CDS             11..625
                     /gene="SRY"
                     /codon_start=1
                     /product="sex-determining region Y protein"
                     /protein_id="QBA69872.1"
                     /translation="MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQ
                     CETGENSKGNVQDRVKRPMNAFIVWSRDQRRKMALENPRMRNSEISKQLGYQWKMLTEA
                     EKWPFFQEAQKLQAMHREKYPNYKYRPRRKAKMPPKNCSLLPADPASVLCSEVQLDNRL
                     YRDDCTKATHSRMEHQLGHLPPINAASSPQQRDRYSHWTKL"
ORIGIN
        1 atgcaatcat atgcaatcat atgcttctgc tatgttaagc gtattcaaca gcgatgatta
       61 cagtccagct gtgcaagaga atattcccgc tctccggaga agctcttcct tcctttgcac
      121 tgaaagctgt aactctaagt atcagtgtga aacgggagaa aacagtaaag gcaacgtcca
      181 ggatagagtg aagcgaccca tgaacgcatt catcgtgtgg tctcgcgatc agaggcgcaa
      241 gatggctcta gagaatccca gaatgcgaaa ctcagagatc agcaagcagc tgggatacca
      301 gtggaaaatg cttactgaag ccgaaaaatg gccattcttc caggaggcac agaaattaca
      361 ggccatgcac agagagaaat acccgaatta taagtatcga cctcgtcgga aggcgaagat
      421 gccgccgaag aattgcagtt tgcttcccgc agatcccgct tcggtactct gcagcgaagt
      481 gcaactggac aacaggttgt acagggatga ctgtacgaaa gccacacact caagaatgga
      541 gcaccagcta ggccacttac cgcccatcaa cgcagccagc tcaccgcagc aacgggaccg
      601 ctacagccac tggacaaagc tgtag
//
LOCUS       MK011514                 625 bp    mRNA    linear   PRI 11-FEB-2019
DEFINITION  Homo sapiens isolate P2-7 sex-determining region Y protein (SRY)
            mRNA, complete cds.
ACCESSION   MK011514
VERSION     MK011514.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Functional characterization of SRY variants in Azoospermic patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 625)
  AUTHORS   Pathak,D. and Dighe,R.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) Department of Molecular Reproduction
            Development and Genetics, Indian Institute of Science, near D Gate,
            Bangalore, Karnataka 560012, India
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /isolate="P2-7"
                     /isolation_source="azoospermic patient"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /sex="male"
                     /tissue_type="testis"
                     /country="India"
                     /PCR_primers="fwd_name: sry2f, fwd_seq:
                     cggatccttgacaatgcaatcatatgcttc, rev_name: sry2r, rev_seq:
                     cctcgagtcctacagctttgtccag"
     gene            1..625
                     /gene="SRY"
     CDS             11..625
                     /gene="SRY"
                     /codon_start=1
                     /product="sex-determining region Y protein"
                     /protein_id="QBA69871.1"
                     /translation="MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQ
                     CETGENSKGNVQDRVKRPMNAFIVWSRDQRRKMALENPRMRNSEISKQLGYQWKMLTEA
                     EKWPFFQEARKLQAMHREKYPNYKYRPRRKAKMLPKNCSLLPADPASVLCSEVQLDNRL
                     YRDDCTKATHSRMEHQLGHLPPINAASSPQQRDRYSHWTKL"
ORIGIN
        1 atgcaatcat atgcaatcat atgcttctgc tatgttaagc gtattcaaca gcgatgatta
       61 cagtccagct gtgcaagaga atattcccgc tctccggaga agctcttcct tcctttgcac
      121 tgaaagctgt aactctaagt atcagtgtga aacgggagaa aacagtaaag gcaacgtcca
      181 ggatagagtg aagcgaccca tgaacgcatt catcgtgtgg tctcgcgatc agaggcgcaa
      241 gatggctcta gagaatccca gaatgcgaaa ctcagagatc agcaagcagc tgggatacca
      301 gtggaaaatg cttactgaag ccgaaaaatg gccattcttc caggaggcac ggaaattaca
      361 ggccatgcac agagagaaat acccgaatta taagtatcga cctcgtcgga aggcgaagat
      421 gctgccgaag aattgcagtt tgcttcccgc agatcccgct tcggtactct gcagcgaagt
      481 gcaactggac aacaggttgt acagggatga ctgtacgaaa gccacacact caagaatgga
      541 gcaccagcta ggccacttac cgcccatcaa cgcagccagc tcaccgcagc aacgggaccg
      601 ctacagccac tggacaaagc tgtag
//
