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Introduction to Oxford Nanopore Technology data analysesThe South Green/i-trop Platform organizes a 5-days course at CERAAS (Thies, Senegal) from November 8 to 12, 2021. The goal of this course is to introduce nanopore sequencing using Oxford Nanopore Technologies (ONT). It included a web lab training for library preparation and bioinformatics training for analysis of ONT data. It specially focused on the following aspects : basecalling, reads quality control, assemblies and polishing/correction, contig quality and structural variants detection. This course is directed towards molecular biologists and scientists who want to apply Oxford Nanopore Sequencing technology in their labs based in West Africa. |
Prerequisites
Linux and knowledge of NGS formats
Program
- Introduction to the principles of the technology (kits, flowcell)
- Principles of basecalling
- Handling mapping and assembly tools suitable for ONT data
- How to polish and correct an assembly
- Detecting structural variations with long reads
Learning objectives
- Understanding limits and advantages of ONT technology
- Manipulating ONT data on a virtual machine on jupyter environment
- Handling mapping, assembly, polishing tools and be able to analyse your own data
- Detecting structural variations using long reads